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Apr 19, 2021 · The silicon cap of the vial was closed and conditioned for 250 °C for 30 min by its insertion into the GC injection port under helium atmosphere. The extraction head was inserted into the headspace bottle and adsorbed for 30 min at room temperature (22 °C).

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Exchange gas in the headspace of the Baltch tube of media with 80% H 2 -20% CO 2 gas at 5 psi. The medium must be colorless before inoculating. If a hint of resazurin is noticeable (see note below), add 0.1 mL reducing agent. Open thawed vial according to enclosed instructions or visit www.atcc.org for instructions.

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Sep 21, 2021 · For sample preparation, 4 g of each sample were put in 45 ml airtight glass vials and sealed with a screw cap with Poly (1,1,2,2-tetrafluoroethylene) (PTFE)/silicone septum. To reach the headspace equilibrium, each vial was kept at 30°C for 30 min and analysed at 22 ± 2°C and 50 ± 5% relative humidity (RH).

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