May 31, 2019 · Non-specific adsorption (NSA) is a persistent problem that negatively affects biosensors, decreasing sensitivity, specificity, and reproducibility. Passive and active removal methods exist to remedy this issue, by coating the surface or generating surface forces to shear away weakly adhered biomolecules, respectively.

Sep 5, 2014 · When investigating the influence of the solvent composition on adsorption, it is recommended to test simultaneously the use of different vial types (i.e. glass versus polypropylene vials). The probability of the analyte to adhere to a specific surface is strongly related to its charge state and consequently to the pH of the sample.

Apr 15, 2023 · The adsorption of analytes to metallic surfaces is referred to as nonspecific as it may take place in any exposed metallic surface in the flow path. As expected, there is a direct relationship between metal surface area and NSA, where higher analyte losses are seen in components that have higher metallic surface area [2].

Jan 28, 2017 · The adsorption process is a surface phenomenon in which the adsorbate is accumulated on the adsorbent surface. When a solution containing absorbable solute comes into contact with a solid with a highly porous surface structure, liquid–solid intermolecular forces of attraction cause some of the solute molecules from the solution to be concentrated or deposited on the solid surface [46–48].

Mar 1, 2019 · The glass surface can be influenced by additional factors such as washing conditions or depyrogenation treatment. 19 Our experiments demonstrated a lesser extent of fogging for unwashed vials and washed/air-dried vials compared to the depyrogenated vials (depyrogenation temperature of 250°C), in line with the observation reported by Abdul

Surface modification of silicon-based devices with polyethylene glycol (PEG) can be performed by physical adsorption, covalent immobilization such as grafting and chemical coupling, or gas phase treatment (plasma treatment or deposition) . Although surface modification efforts have proved successful, however, they do not guarantee conformal

Acidic compounds and neutral compounds mainly adsorb to glass and PP containers by hydrophobic adsorption. Therefore, an organic solvent (methanol, acetonitrile, etc.) or non-ionic surfactant is added to the sample solution to reduce adsorption. Although dependent on the logP of the target compounds, adding 10 to 50 % organic solvent or around

Apr 1, 2021 · Uncoated glass disc samples were used as controls. Adsorption and retention data were reported in ng/cm 2. Statistical Analysis. The effect of surface coating and pH on protein adsorption and retention, both in the presence and absence of PS80, was analyzed using two-way ANOVA followed by post-hoc Tukey's comparison test.

Purpose: To evaluate the adsorption effect of a typical basic drug on the surface of glass autosampler vials. Method: Nortriptyline was chosen as a model compound to demonstrate the adsorption effect. The adsorption of this compound in autosampler vials was monitored using LC/MS/MS analysis.

or into an ICP-MS/atomic adsorption source, or into a cuvette or NMR tube for further characterization. The aim of sample preparation is to provide a sample aliquot that (a) is relatively free of interferences, (b) will not damage the column or instrument and (c) is compatible with the intended analytical method.

Aug 1, 2021 · With the conventional system/column, analyte adsorption results in fewer molecules passing through the chromatographic system and reaching the detector. However, with the HST system/column the inert barrier appears to have prevented analyte loss due to adsorption and thus increasing the number of analyte molecules reaching the detector. 3.3.

Feb 23, 2015 · PDF | On Feb 23, 2015, Wei Li and others published The role of methanol addition to water samples in reducing analyte adsorption and matrix effects in liquid chromatography-tandem mass

Aug 20, 2020 · The longer a peptide or protein is stored in solution the more analyte binding can occur and the lower the recovery. Temperature also influences the kinetics of this binding and influences the extent that it occurs, with adsorption occurring fastest at higher temperatures [5]. The analyte concentration and sample volume will also influence

Jun 1, 2022 · Analyte adsorption was greatest at acidic pH due to the positive charge on the metal oxide surface. Analyte recovery increased when a series of injections was performed; this effect is known as

Apr 10, 2015 · During the preparation and detection process, any experimental apparatus in contact with analytes may cause surface adsorption, including: storage containers, pipette tips, microfiltration membrane filters, and sample vials, as well as sample flow path and sample loop [14], [16], [17], [18]. Therefore, it is important to assess and eliminate