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SPME fiber extraction occurs while the headspace vial is warmed within the 30°C incubator. In our experiments, the fiber was exposed to the headspace for 45 min prior to desorption into the GC/MS inlet. Thermal desorption was performed in a 250°C PTV inlet, with a Siltek Metal Liner (2 mm ID × 2.75 OD × 120 mm Length).

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Direct Growth of Bacteria in Headspace Vials Allows for

Mar 20, 2018 · The ability of SPME-GCMS to analyze bacterial culture directly grown in headspace vials quickly provides a high throughput screening strategy for the study of microbial produced VOCs. This work will support the characterization of microbial diversity in VOC production, as well as enable the development of systems with defined VOC profiles.

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