Using a pipette, wash down any sand that may have stuck to the sides of the column. Drain any additional solvent until the sand is dry, but not down to the silica gel layer. Figure 1. The proper setup for a column chromatography experiment prior to the addition of the sample. 3. Adding the Sample to the Column

or 2-mL vials in full-height plate format. An optional sample organizer increases the capacity of the system to as many as 22 microtiter plates (21 in the sample organizer and one in the sample manager), or eight vial racks (seven in the sample organizer and one in the sample manager). Aijiren Technology® Empower™ chromatography software or MassLynx™ mass

Jun 27, 2006 · Autosampler and software (CTC Combi PAL and PAL cycle composer software version 1.5.0; CTC Analytics); the configuration comprises an agitator-incubator oven, a 98-sample tray for 2.0-ml vials, a

the column. However, if you see precipitate in the sample that might clog the column, centrifuge the sample at 10 000 x g for 1 min to remove the precipitate before applying the sample to the column. XImmediately after the sample has entered the resin, wash the column with 10 bed volumes (6 ml) washing buffer.

May 29, 2013 · Note: A five-fold dilution is one part sample and four parts deionized water. 3. Fill vials at least halfway and cap (~0.6 mL). The cap must fit entirely into the vial when pressed down so that is flesh with the top of the vial. Caps should be placed into the vial with the white filter end touching the fluid inside the vial.

I. Ion Exchange Chromatography 1. Add 0.2ml Equilibration Buffer to the Protein Extract vial. Periodically vortex the tube until the protein completely dissolves. 2. Centrifuge the tu be for 2 minutes at maximum speed in a microcentrifuge to remove the froth. 3. Clamp the Cationic Chromatography Column in an upright position to the stand. 4.

1.1 Refill Wash Vial a. Check and refill wash vials on the injector tower (Fig. 1). The volume of solvent should be above the minimum level specified on the wash vial. b. Default wash vials: A: dichloromethane (DCM); B: methyl tert-butyl ether (MTBE). Your method specifies which wash vials will be used.

10000-µL sample loop, large sample volume (LSV) needle, and sample tray for 10-mL vials • Air needles – Six types, each for a different type of well/vial plate, for pressurizing sample vials of varied heights during aspiration Tubing guide Wash/waste Condensed water/leakage Cooling cover (with cooler option)

first vial and subsequent vials in the sample table, 1. Location – location of sample in autosampler tray. 2. Sample Name – name of sample. 3. Method Name – select method. 4. Inj/Volume – number of injections per vial. 5. Sample Type – Sample/Calibration/Control - typically set to “Sample”. iii.

Protocol 2: Preparing N-dimethylaminomethylene (DMAM) methyl esters of amino acids 1. Combine amino acid with 1:1 ratio of Methyl-8® Reagent to acetonitrile in a Reacti-Vial Small Reaction Vial. 2. Cap vial and heat at 100°C for 20 minutes or until dissolution is complete. 3. Analyze by gas chromatography.

Prepare the top mark and the starting line the same way as before. Make four evenly spaced ticks on the plate, making sure that the outer marks are at least 0.5 cm away from the edges. Draw an exact copy of the plate in your lab notebook and label the tick marks as ‘aspirin’, ‘acetaminophen’, ‘caffeine’, and ‘unknown’.

Autosampler Vial, Clear Chromatography Lab Vial, HPLC LC GC 1.5 ml Wide Opening Short Screw-Thread Sample Vial with Write-on Spot, Clear, 12x32mm, 8-425 Top Autosampler Vials | Aijiren Tech Scientific For use with snap caps or aluminum crimp seal closures, these high-quality 11mm amber glass vials are 2mL, 12x32mm and fit most brands of autosamplers.

Dec 04, 2014 · The sample (5 μl), followed by 1.5 μl of the reagent, is loaded to ensure that the sample loop is completely filled with the sample. Subsequently, the sample is injected for 2 min at 0.75 μl/min.

as dialysis, long centrifugations and slow chromatography. Below are some protocols and tips which help me to achieve this goal. My approach is based on classical combination of ion-exchange, hydrophobic and size-exclusion chromatography for natively (no tags) over-expressed proteins.

(11) Capillaries, one glass dispenser vial containing 100 sodium-heparinized capillary tubes (5 uL). Reorder box of 20 vials (50 capillary tubes/vial), Cat. No. 195-1053, Bio-Rad Laboratories, Clinical Division.