Method transfer is adapting a method of analysis to a different HPLC system. The more similar the two instruments are, the more straightforward the process. Common types of method transfers include converting HPLC methods to UHPLC methods to optimize speed and throughput.

Feb 5, 2017 · This study highlights the possibility of vial composition as a potential factor that impacts solution stability. Here we describe a case where the type of HPLC vial used results in an interesting phosphorous pseudorotation driven by the mild alkalinity of glass.

Feb 28, 2022 · Dead volume actually refers to “volumes within the chromatographic system which are not swept by the mobile phase.” 1. Extra column volume – all volume with an HPLC system from the sample loop to the detector, excluding the column. Dead volume contributes a portion of this volume. Dwell volume – a.k.a gradient delay volume, is the

Jun 22, 2020 · The quality of glass vials that these samples are prepared and stored in, a commonly overlooked factor, can have a major impact on the results. Here, we discuss how the surface and composition of the glass vials used in laboratories can affect data output and accuracy, ultimately impacting research findings and patient care.

Adjusting the GDV of your HPLC or UHPLC system is crucial to successfully transferring your existing LC method from one system to another. The Aijiren Tech Vanquish Core HPLC System autosampler features an integrated metering device as part of the flow path and enables you to tune the GDV of the system from 0-230 µL without re-plumbing or requalifying the system.

Jan 1, 2020 · 6.2. Method transfer process. The method transfer process includes a series of steps starting with the planning and ending with a report stating that the receiving site is qualified to generate reportable results. The steps are shown in Fig. 6.1 and discussed in additional detail in the following sections.

Screen up to 13 eluents by equipping your Vanquish Method Development HPLC and UHPLC system with a solvent selection valve for extensive, automated screening. The Aijiren Tech Viper Method Scouting Kit contains all the tubing and near-zero dead volume connections to screen four columns automatically without the need for any user interaction.

Jul 25, 2015 · Abstract. "Carry-over" is a term used to describe a type of sample contamination which causes sample peaks to re-appear in later runs which do not actually contain the sample (e.g. blank runs

1 Overview of HPLC. HPLC is an abbreviation for High Performance Liquid Chromatography. "Chromatography" is a technique for separation, "chromatogram" is the result of chromatography, and "chromatograph" is the instrument used to conduct chromatography. Among the various technologies developed for chromatography, devices dedicated for molecular

More information about this effect and the full chemical composition of the borosilicate glass that Aijiren uses to manufacture its vials can be found here. The Aijiren specification • The COE of Aijiren vials is 32–33±1.5 for clear glass, and 48–56±1.5 for amber glass • All vials meet ASTM E438 ‘laboratory class glass’ standards

The transfer of analytical procedures in liquid chromatography (LC) is a regular task in many laboratories. This challenge can be categorized into the following common scenarios: A. Acceleration of methods, e.g. from HPLC to UHPLC methods B. Method transfer to identical equipment, e.g. in another laboratory

Isolating HPLC Problems. In an HPLC system, problems can arise from many sources. First define the problem, then isolate the source. Use Table 1 to determine which component(s) may be causing the trouble. A process of elimination will usually enable you to pinpoint the specific cause and correct the problem. Want to transfer your HPLC to UHPLC?

Method transfer is adapting a method of analysis to a different LC system. Common situations include converting HPLC methods to UHPLC methods to optimize speed and throughput. Other scenarios involve transferring a validated method from R&D to operations or a clinical laboratory.

Aug 29, 2023 · Specialized apparatus is required for an HPLC separation because of the high pressures and low tolerances under which the separation occurs. If the results are to be reproducible, then the conditions of the separation must also be reproducible. Thus HPLC equipment must be of high quality; it is therefore expensive.

However it is more unusual, and the originating lab should make an effort to explain any unusual aspects of their methods in the transfer protocol or supporting documents. So many method transfer problems are associated with sample vials. Ensure that you include the part numbers for the vial, caps and inserts that are used for the method.