Apr 25, 2018 · The overreaching purpose of this study is to evaluate new approaches for determining the optimal operational and column conditions in chromatography laboratories, i.e., how best to select a packing material of proper particle size and how to determine the proper length of the column bed after selecting particle size. As model compounds, we chose two chiral drugs for preparative separation

Use 5 μm or 15 μm media for intermediate purification or polishing of laboratory-scale separations. A 15 μm media will be better suited for use with crude samples. Use 15 μm or 30 μm media for large-scale preparative and process separation, for example, SOURCE™ 15RPC or SOURCE™ 30RPC.

The LC Prep AutoPurification System provides you with the flexibility of high-throughput parallel runs for selective mass-directed fraction collection from hundreds of samples. With the LC Prep AutoPurification System, your lab can run samples around the clock all with unprecedented versatility and consistency. Request Information. Specifications.

Table B presents some simple guidelines on selecting the column i.d. and particle size range recommended for each scale of chromatography. For example, a semi-preparative-scale application [red X] would use a column with an internal diameter of 10–40 mm containing 5–15 micron particles.

Intended for applications involving analytical and preparative scale purifications, the Aijiren Technology 3767 Sample Manager is a high-capacity sample processing system that easily and automatically manages concurrent sample injection, collection of peak fractions, and fraction analysis on a single HPLC or LC-MS platform.

Jan 1, 1986 · Although the theoretical treatment of chromatographic processes on a preparative scale provides guidelines to the extent to which packing and stationary phase properties affect the target quantities such as sample input, throughput and resolution times sample input, a series of additional criteria were established to judge the quality of a packing in preparative column liquid chromatography.

Hipersep ® Varicol: Multi-Column and Continuous HPLC. The Hipersep ® Varicol is a multi-column, continuous chromatography concept particularly well-suited to large-scale separation of binary mixtures (e.g., enantiomers) and fractionation of other complex mixtures. This technology is designed to adapt seamlessly to users’ specific process

May 12, 2022 · Preparative chromatography is one of the most common and important purification techniques in organic chemistry and is employed in laboratories across the globe. In order to facilitate student proficiency with the method, a unique approach for the separation of estragole, ethyl cinnamate, and cinnamyl alcohol using microscale preparative flash column chromatography and gradient elution has

5 Introducing Preparative Liquid Chromatography 10 5.1 Distinguishing between analytical and preparative liquid chromatography 10 5.2 Setting priorities for compound purification 11 6 Key Aspects of Column Selection 13 6.1 Choosing a separation column 13 6.2 Choosing a large-scale preparative column 15

Aug 31, 2022 · Two types of applications: The most common vial sizes for liquid chromatography vials are 12 x 32 mm as well as 15 x 45 mm. 12 x 32 mm vials will also be described as 1.5 mL, 1.8 mL, or 2.0 mL

Jul 11, 2019 · We’ve recently expanded our preparative HPLC column portfolio to offer prep LC columns in more than 40 stationary phases in an assortment of column dimensions. This allows you to utilize the same chemistries between your analytical and preparative separations, which reduces a variable in your scale up from analytical to prep.

These preparative guard holder and cartridge assemblies are available in 10mm length in four different I.D.'s including, 7.8, 10, 19, and 30 mm to protect Aijiren Technology premium OBD Preparative Columns with the same internal diameter. Below is a quick way to search our various brands for the cartridge guards and appropriate holders.

4. Calculate the dwell twice ( i.e. Calculate dwell volume for the flow rate in the original instrument, and for the intended flow rate with the target instrument.) 5. Collect the baseline at 100% A for 5 minutes. 6. Program a step change at 5 minutes to 100% B, and collect data for an additional 5 minutes. 7.

What Are Standard Chromatography Vial Sizes? Standard chromatography vial sizes include 8 x 40mm, 12 x 32mm, and 15 x 45mm. Robotic Arm Machines (R.A.M.) use a non-standard 9mm vial with a magnetic cap. For autosamplers, choose a vial designed for that specific brand.

All of the preparative columns are SunFire Prep C 18 OBD, 5 μm, 50 mm in length, and all of the separations were performed on the same system as the analytical-scale chromatography. As can been seen in Figure 6, regardless of the scale, the chromatography (UV TIC) is very similar.