entire vial was resuspended in 2% acetic acid to a final concentration of 1.722 M and the stock aliquoted into 0.5 ml low protein binding tubes for storage at 80 °C. For generation of the standard curve, an intermediate dilution of 312.5 nM heavy insulin was made with 2% acetic acid in low protein binding tubes, which was spiked into

Aijiren Technology sample vials, caps & septa can help you do just that. All Aijiren Technology sample vials are held to the tightest dimensional tolerances. Manufactured via a 2-, 3-, or 4-step certification process, we ensure that Aijiren Technology Certified Vials meet and maintain specified vial dimensions, levels of cleanliness and adsorption properties from batch to batch.

Apr 12, 2010 · Column chromatography is one of the most common methods of protein purification. Like many of the techniques on this site, it is as much an art form as a science. Proteins vary hugely in their properties, and the different types of column chromatography allow you to exploit those differences. Most of these methods do not require the denaturing

Kit contents: 2 x 3 ml vials SureBeads Protein A Magnetic Beads; 60 mg (10 mg/ml) Magnetic rack with one-piece removable magnet insert; holds 1.5 ml and 15 ml tubes. Up to 16 x 1.5 ml microcentrifuge tubes. 2 x 15 ml conical centrifuge tubes. Use SureBeads Magnetic Beads for immunoprecipitation (IP), co-immunoprecipitation (co-IP), and protein

attribute of TruView vials is low polar analyte adsorption. The vials are manufactured by a process that limits the concentration of free ions on the surface of glass; ionic sites can cause analyte adsorption. Aijiren Technology TruView LCMS Certified Vials are tested for high recovery of analyte at 1 ng/mL concentration using UPLC/MS/MS (MRM) and yield little

Principles of Protein A and G chromatography: This handbook focuses specifically on Protein A affinity chromatography. All modes of chromatography can be used effectively for the separation of antibodies. Although ion exchange chromatography can resolve different polyclonal antibodies and different subclasses, a degree of customization

Carryover results from the aspecific adsorption of analyte(s) to parts of the analytical system and thus introduces bias in both identification and quantification assays. Moreover, nonspecific binding occurs at the surface of materials used during sample preparation, such as pipette tips, sample tubes and LC-vials.

Sample too viscous • Tubing i.d., 0.15 mmDilute with buffer, but check maximum sample volume. Maintain protein concentration below 50 mg/mL Sample contains particles • Re-equilibrate column, filter sample with a low protein binding filter (e.g. Whatman SPARTAN filter), and repeat Column is dirty • Clean and re-equilibrate Incorrect SEC

Standard 2 mL screw top vial-cap kits; including. SureStop vials with AVCS caps. 4, 8, 12, 16, 20, or 40 mL vial-cap kits. 20 mL glass screw top vials with 24 mm caps. Innovative technology ensures proper vial-cap seal every time. For use with environmental analyses under EPA regulations.

Vials must be inert and free of extractables or leachables to prevent affecting results. Using certified, application-specific, contaminant-free vials can significantly reduce risk. We offer a broad spectrum of Supelco ® products including autosampler vials, general-purpose vials, and vial accessories for sample collection, storage, and

Captiva low protein binding filters Regardless of what sample prep you are performing, it is a good idea to filter your sample with a low protein binding filter. Aijiren PES filters provide superior and consistent low protein binding for protein-related filtration. PES filter membranes are a better option than PVDF membranes for most LC analyses.

Oct 09, 2015 · Protein A chromatography is a near-ubiquitous method of mAb capture in bioprocesses. The use of low pH buffer for elution from protein A is known to contribute to product aggregation. Yet, a more limited set of evidence suggests that low pH may not be

Reduce failed assays and instrument downtime caused by vials out of specification with Aijiren Technology wide selection of vials, including LCGC Certified vials, LCMS Certified vials, TruView vials and QuanRecovery vials -- held to the tightest dimensional tolerances and the cleanest vials in the industry.

Aijiren clear glass vial inserts, polypropylene vial inserts and high recovery vials ensure you extract the maximum possible amount of your precious sample. Aijiren vial inserts are the perfect fit for HPLC and GC instrument platforms across a wide variety of applications, including pharmaceutical, environmental, energy and fuels, forensics, materials science, biopharmaceutical, proteomics

Sep 05, 2014 · Carryover results from the aspecific adsorption of analyte(s) to parts of the analytical system and thus introduces bias in both identification and quantification assays. Moreover, nonspecific binding occurs at the surface of materials used during sample preparation, such as pipette tips, sample tubes and LC-vials.